Wolves (Canis lupus) can be primary predators of beavers (Castor canadensis), but little is known about wolf-beaver dynamics. We identified kills from 1 wolf (V009) of the Ash River Pack in Voyageurs National Park from 1 April to 5 November 2015 to provide direct estimates of wolf pack kill and predation rates of beavers. We documented 12 beaver kills by V009 during the 2015 ice-free season and estimated V009 killed 22 beavers during this period. Based on the number of beavers killed by V009, we estimated the Ash River Pack removed 80–88 beavers (kill rate of 0.085–0.095 beavers/wolf/day), which was 38–42% of the beaver population in their home range during the ice-free season. Even with this substantial level of predation in 2015, the beaver population in the Ash River Pack home range increased by an estimated 43% in 2016, which suggested dispersal from more densely populated adjacent areas likely compensated for the effects of wolf predation. We have presented the first direct estimate of wolf kill and predation rates on beavers, but more research is necessary to understand how wolf predation affects beaver populations under a variety of conditions.